R2R-Biofunctionalization/ Microspotting
JOANNEUM RESEARCH Forschungsgesellschaft mbH
JOANNEUM RESEARCH is a professional leader of innovation and provider of technology, performing cutting-edge research at the highest international standards for 30 years. In MATERIALS, a team of around 80 researchers provides interdisciplinary solutions across the entire value chain – from the idea to the prototype - in the fields of * Microfluidic Lab-on-a-Foil Systems * Roll-to-Roll Micro-/ Nanostructuring * Chemo-/ Biofunctionalization * Additive Manufacturing * Laser-/ Plasmaprocessi
MedPhab partner
INTEGRATION [Surface activation] Array spotting
IN4 Surface activation
Array spotting Bio-reagent dispense
in vitro diagnostics others
Technology provider Supplier Manufacturer Integrator R&D
Medium scale Micro-Spotting system with stop&go R2R integration and highly flexible fiducial recognition. The system enables the rapid, multiplexed bio-functionalization of practically any substrate with DNA or proteins by microspotting from up to eight independent piezo dispense capillaries.
Micro Spotter make & type Scienion AG sciflexARRAYER12
R2R process workflow Automatic Stop&Go Integration
Fiducial recognition yes
Spotting & Curing Area (L x W) 38 x 38 cm
No. of piezo dispense capillaries up to 8
Dispense Volume 220< V <600 pL
Positioning accuracy <5 um
Spot diameter (typical) 25< D <500 um
Spotting patterns Freely designable
Spot spacing (typical, adjustable) 250 um
UV curing intensity @substrate (254 nm) 27.5 mw/cm2
Reagents (aqueous solution) DNA, Proteins
Immobilisation enhancement agent (optional) Scipoly 3D (Scienon AG)
Temperature & humidity control optional
Humidity range 0< H <85 %
Substrate surface materials include polymers, glass, Si, SiO2, Au, hydrogels, …
4 5
500 chips/day (multiplexed dispense)